A simple, precise, accurate and reproducible UV spectrophotometric method (1st derivative) has been developed for simultaneous estimation of Celecoxib and Diacerein in bulk and combined dosage form using a double beam UV-Visible spectrophotometer using Methanol as solvent and spectra converted to 1st derivative and absorbance measured at two wavelengths i.e. 251.8 nm (Zero crossing point of Celecoxib) and 254.6 nm (Zero crossing point of Diacerein). The linear correlation was obtained for Celecoxib and Diacerein in the concentration ranges of 4-20 μg/ml and 2-10 μg/ml with correlation co-efficient 0.999 and 0.999 respectively. The mean recoveries were found to be 100.49% & 100.42% for Celecoxib and Diacerein respectively. Since the 1st derivative method has been developed and validated to ensure that the performance characteristic of the method meets the requirements for the intended analytical applications.
Loading....